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Fluorescence Study Of Dyes
Start of Term Paper
1. Introduction
Coumarin 1 and Sodium fluorescein are two dyes which absorb and emit light in the visible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficient .... Middle of Term Paper
... the acceptor the fluorescein. When the donor is excited, it is naturally decaying to the ground state. However in presence of an acceptor, this process is enhanced trough energy transfer. We will distinguish between collisional energy transfer and dipole-dipole transfer. From here we will try to determine the quenching constant and the distance at which decay and energy transfer are equally probable, as well as prove that Stern-Volmer's law and Förster's theory are obeyed.
2. Results
2.1. Electronic Absorption Spectra
We made up stock solutions for both salts and diluted them down to use in the UV/vis spectrometer.
Coumarin 1
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| Number of Words: 815 |
Approximate Pages: 3 |
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