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Proteins are the macromolecules that are responsible for most of the bodily functions. By investigating an individual protein, one can be able to understand the functions and structure of an organism. Before this can be done, protein has to b ....

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... of protein extract. An electrotransfer on PVDF membrane was done after the gel electrophoresis. These migrations of proteins based on their molecular weight of three solutions. Using the polyacrilamide electrophoresis, proteins were separated based on their molecular weight. Extraction buffer did not produce protein bands. Proteins with huge molecular weight did not travel far from the gel while low molecular weight proteins traveled long distance . Overall banding contrast was compared between same sample with different transferred volumes. The protein bands of ADH were closely related to some of the bands of liver protein extrac ...