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Chromatography
Start of Term Paper
is a separation technique in which the mixture to be separated is dissolved in a solvent and the resulting solution, often called the mobile phase, is then passed through or over another material, the stationary phase. The separation of the or .... Middle of Term Paper
... size. These differences allow the mixture components to be separated from each other by using these differences to determine the transit time of the solutes through a column.
Simple liquid consists of a column with a fritted bottom that holds a stationary phase in equilibrium with a solvent. Typical stationary phases (and their interactions with solutes) are: solids (adsorption), ionic groups on a resin (ion-exchange), liquids on an inert solid support (partitioning), and porous inert particles (size exclusion). The mixture to be separated is loaded onto the top of the column followed by more solvent. The different components in the sampl ... |
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