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Green Fluorescent Protein Purification
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Green Fluorescent Protein Purification
Green Fluorescent Protein (GFP) is a relatively new biochemical tool with a wide range of applications in current research. The natural function of GFP is to facilitate the bioluminescence of jellyfish an .... Middle of Term Paper
... fluorophore returns to its original state. This cycle allows the fluorophore to reliably emit a high yield of light with no outside influence other than the incoming photon. So GFP is basically a stable little (27-30 kD) green light bulb that can be tacked on to other proteins at either the N- or C-terminus without a distortion of its compact beta-can structure. Biochemists now frequently exploit GFP’s unique structural and physical characteristics to visualize various cellular activities.
One such application of GFP is to understand the role of a hormone, T3, in the translocation of thyroid hormone nuclear receptors (TRs).* In this pro ... |
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