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Determination Of An Unknown Amino Acid From Titration
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Abstract
Experiment 11 used a titration curve to determine the identity of an
unknown amino acid. The initial pH of the solution was 1.96, and the pKa’s
found experimentally were 2.0, 4.0, and 9.85. The accepted pKa values were
found to be 2 .... Middle of Term Paper ... The amine group (NH2) is Lewis
base because it has a lone electron pair which makes it susceptible to a
coordinate covalent bond with a hydrogen ion. Also, the carboxylic group is a
Lewis acidic because it is able to donate a hydrogen ion (Kotz et al., 1996).
Other forms of amino acids also exist. Amino acids may exists as acidic or
basic salts. For example, if the glycine reacted with HCl, the resulting amino
acid would be glycine hydrochloride (see fig. 2). Glycine hydrochloride is an
example of an acidic salt form of the amino acid. Likewise, if NaOH were added,
the resulting amino acid would be sodium glycinate (see fig. 3), an ex ... |
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